This method is free of interference from Hemoglobin, Bilirubin or Lipemias. The samples can be run without deproteinization or dilution. The methodology uses two reagents and is free of interference in cases of newborn icterus due to the low concentration during this period, in patients undergoing hemodialysis or where ascorbic acid is present at elevated levels, such interferences being very common with the traditional Jaffe methodology
The reaction :
Below are two graphics. The 1° one is the traditional Jaffe reaction and the interference of unconjugated/conjugated bilirubin and hemoglobin occurred during the production of the Creatinine reaction. On the 2° graphic with the Synermed method after the 1° incubation with R1, the sample is totally free of interferences and, after the addition of the R2 the reaction curve is produced without interference.
Traditional Kinetic Jafffe
Synermed VI 560 Creatinine