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BUN Interference Comparison Urea is one of the few serum analytes which can be measured using ultraviolet detection without serious chromatic interference. The reason can be seen from the molar concentration at the upper limit of normal. Urea is one of the most plentiful molecules in serum. Additionally, two molecules of NADH are oxidized per molecule of urea. Thus, the signal [chromophore absorbance] to noise [serum background absorbance] is very favorable, relative to other serum analytes.


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