Principles of the Reaction
Creatinine reacts with picrate ions in an alkaline solution to form a red tautomer of creatinine picrate which can be quantitated spectrophotometrically at 500 - 530 nm.
OH-
Creatinine + picric acid -> Creainine-picrate complex
(red)
The Synermed creatinine reagent system utilizes an alkaline picrate solution with an added compound to decolorize endogenous bilirubin, thus eliminating bilirubin interference in the method. The creatinine reaction is measured kinetically to virtually eliminate interferences from Jaffé positive substances.
Analytical Range
Most applications of this procedure have demonstrated linearity to 24 mg/dL (2122 µmol/L).
Limitations of the Procedure
- Conjugated bilirubin levels up to 30 mg/dl have been tested in the Synermed laboratory and produced no interference in this method.
- Unconjugated bilirubin levels up to 30 mg/dl have been tested in the Synermed laboratory and produced no interference in this method.
- Hemoglobin levels up to 1000 mg/dl have been tested in the Synermed laboratory and produced no interference in this assay.
- Lipemia levels up to 1000 mg/dl (as Intralipid) have been tested in the Synermed laboratories and produced no interference.
Expected Values
The normal range for creatinine in adult human serum is 0.7-1.5 mg/dL (62-133 µmol/L).
Product Packaging and Storage
Cat No. |
Product Name |
Packaging |
Packaging Volume (mL) |
Storage |
VI060-X |
Creatinine, single reagent |
2x250 mL |
500 |
20-25° C |
VI060 |
Creatinine, single reagent |
6x250 mL |
1,500 |
20-25° C |
VI260-X |
Creatinine, 2 reagent |
1x250 mL + 1x50 mL |
300 |
20-25° C |
VI260 |
Creatinine, 2 reagent |
5x250 mL + 1x250 mL |
1,500 |
20-25° C |
VI261-L |
Creatinine Buffer |
6x1000 mL |
6,000 |
20-25° C |
VI262-L |
Creatinine Picrate Reagent |
6x1000 mL |
6,000 |
20-25° C |
VI560-X |
Creatinine |
1x250 mL + 1x250 mL |
500 |
20-25° C |
VI560 |
Creatinine |
3x250 mL + 3x250 mL |
1,500 |
20-25° C |
VI561-L |
Creatinine Buffer |
6x500 mL |
3,000 |
20-25° C |
VI562-L |
Creatinine Picrate Reagent |
6x500 mL |
3,000 |
20-25° C |
VI560-911 |
Creatinine |
4x50 mL + 4x50 mL |
400 |
20-25° C |
VI560-WK |
Creatinine |
3x25mL + 3x25mL |
150 |
20-25° C |