The Synermed® cholesterol methodology enhances signal to noise ratio as compared to traditional automated methods in two ways. First, the chromophore produced has more than twice the molar absorbance compared to the most commonly used automated methods. Second, the shift in wavelength to the infrared diminishes absorbance due to lipemia, bilirubin and hemoglobin. The overall signal to noise enhancement is about 700% for lipemia. Hemoglobin and bilirubin produce no chromatic interference in the method.
The improved performance relative to chromatic substances is dramatic in the application of the method to HDL cholesterol.